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Title: | Electrokinetic Flow Assisted Dentin Disinfection |
Authors: | Gallucci, German O Nagai, Shigemi Ohyama, Hiroe |
Description: | Background: The bacterial persistence within the root canals and dentinal tubules in infected and necrotic pulp poses a serious health risk, with reinfection of the endodontically treated tooth being one of the main problems of root canal treatment. In the current disinfection method in conventional endodontic treatment, the depth of bacterial penetration cannot be eliminated either by mechanical instrumentation or chemical irrigation, due to the passivity of diffusion and the complex anatomical structures within the root canal system. The aim of the present study was to characterize the role of electrokinetic flow in the disinfection of dentinal tubules. Methods: The study involved five different tiers of experimental design. In the first experiment, the electrokinetic flow assisted infiltration of antimicrobial solution into the 100 µm-thickness dentin slabs were studied using fluorescent microscopy as well as using the change in current as function of time throughout the application of the electrokinetic flow. The second experiment involved in vitro study of bacterial infiltration using E. coli infiltrated dentin specimen. In the third experiment involving ex vivo study of electrokinetic flow application on whole human tooth, the bacterial viability in cathode reservoir before and after the infiltration of antimicrobial solution was studied via bacterial motility monitoring and fluorescent microscopy. The fourth experiment involved ex vivo study of electrokinetic flow application in porcine mandible. The fifth experiment involved the development of a preclinical prototype for electrokinetic flow assisted dentin disinfection. Results: The results of the present study revealed that electrokinetic flow was able to carry the antimicrobial solution throughout the thickness of dentin specimen. This phenomenon was observed via various independent measurements including fluorescence microscopy, confocal laser scanning microscopy, bacterial motility, and ion selective electrodes. In particular, the in vitro experiments showed that the full thickness of the dentin specimen was infiltrated completely at 1200 seconds. In addition, the findings from scanning electron microscopy and confocal laser scanning microscopy revealed a skewed presence of GFP E. coli limited to the inner (canal wall) and the outer surfaces of the root. This finding supports the notion that dentin is not conducive for uniform infiltration of bacteria by the use of centrifugation, due to the complex morphology of collagen and hydroxyapatite pore system. Conclusion: Within the limitations of this study, the use of electrokinetic flow shows this novel technology as a promising model for clinical application in dentin disinfection, with enhanced infiltration of therapeutic solution and the capacity for optimization of time and current. |
URI: | http://lib.yhn.edu.vn/handle/YHN/156 |
Other Identifiers: | Yi, Anna Hwayoung. 2021. Electrokinetic Flow Assisted Dentin Disinfection. Doctoral dissertation, Harvard University School of Dental Medicine. 28541375 https://nrs.harvard.edu/URN-3:HUL.INSTREPOS:37368648 0000-0003-3717-3450 |
Appears in Collections | Y học |
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